Targeted Disruption of
We aim to develop a multi-functional and stimuli-responsive nanovehicles―for a spatially- and temporally-controlled delivery of formulations specific to biofilms. These nanocarriers will be loaded with antimicrobial agents―that will target, in a defined manner, the different major contributors of biofilm formation. This is in line with one of our primary goals—development of novel anti-adhesion therapy.
Molecular forces in bacterial biofilms
Central to the initiation and maturation of biofilms are the cell surface adhesion molecules (adhesins) that mediate initial attachment between the cell and a wide range of surfaces. We use Atomic force microscopy (AFM)-based single-molecule and single-cell force spectroscopy to quantify interaction forces between adhesins responsible for initial attachment and early microcolony formation and relevant surfaces. Our goal is to elucidate mechanisms of initial adhesion by addressing the nanoscale interplay between the properties of the bacterial surface and the underlying substrate.
Rigidity sensing or how adherent cells respond to the stiffness of the underlying substrate is well-known in mammalian cells and is established to influence important biological processes such as stem cell differentiation and cancer cell metastasis. Discovery of rigidity sensing in bacteria however is still rather recent. By quantifying the mechanical forces that govern the interaction of key adhesins with substrates of varying stiffness, we aim to paint a more unified molecular picture of bacterial rigidity sensing.